Jui Gadkari was born in Kalyan a town near Mumbai, father Ketan Gadkari who is a Play Writer, a rhythmist and Mrs. Netra Ketan Gadkari. She has completed her school from Nerul's St. Augustine's High School and Ryan international school and Graduation in Mass Media from Ulhasnagar's CHM college. She holds a PG in advertising and PR and a MBA in marketing from Welingker's institute. She is also the gold medalist of Mumbai university for her graduation.
Jui never thought of acting as her career. It was after her post graduation that the took up with her job as Asst. director in a production house, she realised that she had some interest in this field. her mother insisted her to have a portfolio done and took her to the legendary photographer late. Gautam Rajadhyaksha. He guided her and she had her first portfolio done. she then started with her career with full swing. She always mentions that Gautam played the most important role in shaping and motivating her to meet the right people at the right time and at the right place. Jui is also a trained classical singer and also learns Katthak. She also is an active mountaineer. She is also associated with several NGOs and she regularly visits 'shantivan' a rehab for the leprosy patients.
Jui also worked with White paper communications, a production house as an Asst. director. She portrayed a character named 'kalyani' in Marathi drama 'Pudhcha Paaul'.
Jui is also a female brand ambassador for the 'tobacco free maharashtra'. A mission by pune's dnyaneshwari pratishthan.